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Reagents and ratios for transfection of the DRD2 and TRUPATH biosensors in this protocol
| Value | 10 mM dish |
|---|---|
| Cell Density | 6.5–7.0 × 106 |
| Receptor DNA | 1 μg hDRD2 |
| Gα DNA | 1 μg GαZ TRUPATH |
| Gβ | 1 μg Gβ3 |
| Gγ-GFP2 | 1 μg Gγ1-GFP2 |
| Opti-MEM | 400 μL |
| TransIT-2020 | 12 μL |
