Complete DMEM	Final concentration	Amount
FBS	a10%	50 mL
Penicillin-streptomycin	a100 U/mL-100 $\mu$g/mL	5 mL
L-Glutamine	2 mM	5 mL
DMEM	n/a	500 mL
Total	n/a	560 mL

^aApproximately; Store at 4°C for up to 1 month.