1% Dialyzed FBS DMEM	Final concentration	Amount
dFBS	1%	5 mL
Penicillin-streptomycin	100 U/mL-100 $\mu$g/mL	5 mL
L-Glutamine	2 mM	5 mL
DMEM	n/a	500 mL
Total	n/a	515 mL

^aApproximately; Store at 4°C for up to 1 month.