Poly-L-lysine solution	Final concentration	Amount
Poly-L-lysine (50 mg/mL)	100 $\mu$g/mL	2 mL
Penicillin-streptomycin	100 U/mL-100 $\mu$g/mL	10 mL
Distilled water	n/a	988 mL
Total	n/a	1,000 mL

Sterile filter and store at 4°C for up to 3 months