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. 2022 Apr 1;3(2):101259. doi: 10.1016/j.xpro.2022.101259
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins
Bovine Serum Albumin Fraction V, fatty acid free (BSA) MilliporeSigma Cat# 10775835001
Dopamine hydrochloride Tocris Bioscience Cat# 3548
Droperidol Tocris Bioscience Cat# 5858
Dulbecco’s Modified Eagle Medium (DMEM) Thermo Fisher Scientific Cat# 11995065
Ethylenediaminetetraacetic acid (EDTA) MilliporeSigma Cat# E5134
Fetal Bovine Serum, heat inactivated (FBS) Thermo Fisher Scientific Cat# 10082147
Fetal Bovine Serum, dialyzed (dFBS) Thermo Fisher Scientific Cat# 26400044
Hanks’ Balanced Salt Solution (HBSS) (10×) Thermo Fisher Scientific Cat# 14065056
HEPES MilliporeSigma Cat# H3375
L-Ascorbic acid MilliporeSigma Cat# 795437
L-Glutamine Thermo Fisher Scientific Cat# 25030024
L741,626 Tocris Bioscience Cat# 1003
Methanol Thermo Fisher Scientific Cat# 10141720
Opti-MEM Thermo Fisher Scientific Cat# 31985062
Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122
Premixed phosphate buffered saline (PBS) (10×) MilliporeSigma Cat# P4417
Poly-L-lysine hydrobromide MilliporeSigma Cat# P2636
Potassium hydroxide (KOH) MilliporeSigma Cat# 221473
Prolume Purple NanoLight Technology Cat# 369
Risperidone Tocris Bioscience Cat# 2856
TransIT-2020 Mirus Cat# MIR 5400
Trypsin-EDTA (0.05%) Thermo Fisher Scientific Cat# 25300062
Ziprasidone hydrlochloride Tocris Bioscience Cat# 3085
Experimental models: Cell lines
Human embryonic kidney 293T cell line ATCC Cat# CRL-3216
Recombinant DNA
Olsen et al. (2020) Addgene Cat# 1000000163
Software and algorithms
Prism GraphPad https://www.graphpad.com/
Other
12.5 μLGripTips Pipette Tips for VIAFLO 96/384 Integra Cat# 6453
125 μLGripTips Pipette Tips for VIAFLO 96/384 Integra Cat# 6463
384 Channel Pipetting Heads (5–125 μL) Integra Cat# 6132
BackSeal-96/384, White Adhesive Bottom Seal PerkinElmer Cat# 6005199
Cell Culture Microplate, 384 wells Greiner Bio-One Cat# 781098
Centrifuge 5910 Ri – Refrigerated Centrifuge Eppendorf Cat# 5943000131
Echo 650T Liquid Handler Beckman Coulter Inc. Cat# 001-16078
Nunc EasYDish Dishes, 100 mm diameter Thermo Fisher Scientific Cat# 150464
Nunc EasYDish Dishes, 150 mm diameter Thermo Fisher Scientific Cat# 150468
PHERAstar FSX with BRET2 optic module BMG LABTECH www.bmglabtech.com
VIAFLO 384, 24, 96, and 384 channel handheld pipette Integra Cat# 6031
384 Channel Pipetting Heads (0.5–12.5 μL) Integra Cat# 6131