Fig. 1.

Characterization of wildtype and S100A9 knockout tumor cells. (a) PCR of tumor cell lysates (upper row) and harvested tumor tissue deriving from orthotopically grown breast tumors (lower row). (b) Western Blot of S100A8 and S100A9 regarding the four different tumor cell lines, Hoxb8 cells as positive control. (c) Adhesion/optical density over time as comparison of the four different cell lines. Optical density of 4T1 wt tumor cells is significantly higher as compared to the lower malignant 67NR wt tumor cells. (d) Migration rate comparing the wildtype tumor cells. 4T1 cells have a significantly higher migration rate as compared with 67NR. (e) Relative tumor growth over 20 days revealed no differences within the 4T1 or the 67NR system (for both wt and ko tumor cells in wt and ko mice each), but tumor growth of all 4T1 tumors was faster as compared with 67NR tumors.