Skip to main content
. 2022 Jan 6;20(4):622–636. doi: 10.1158/1541-7786.MCR-21-0149

Figure 1.

Figure 1. NSC59984 constitutively induces ERK1/2 phosphorylation. A, The phosphorylation of ERK1/2 in different cancer cells. Cancer cells were treated with NSC59984 (μmol/L) for 16 hours. B, The phosphorylation of ERK1/2 in SW480 cells treated with NSC59984 (μmol/L) in a dose and time course. C, The phosphorylation of ERK1/2 in SW480 cancer cells treated with NSC59984 (μmol/L) and NAC (mmol/L) for 16 hours. D, The phosphorylation of ERK1/2 in SW480 cancer cells with knockdown of Ras. SW480 cells were transfected with siRNAs (siRNA #1 and #2) to knockdown Ras, followed with NSC59984 (μmol/L) treatment for 16 hours. E, The phosphorylation of ERK1/2 in SW480 cells with knockdown of Raf expression. C-Raf or B-Raf expression was knocked down by siRNA in SW480 cells, followed with NSC59984 (μmol/L) treatment for 16 hours. F, The phosphorylation of ERK1/2 in SW480 cells treated with NSC59984 (μmol/L) and the different kinase inhibitors (μmol/L) as indicated for 16 hours.

NSC59984 constitutively induces ERK1/2 phosphorylation. A, The phosphorylation of ERK1/2 in different cancer cells. Cancer cells were treated with NSC59984 (μmol/L) for 16 hours. B, The phosphorylation of ERK1/2 in SW480 cells treated with NSC59984 (μmol/L) in a dose and time course. C, The phosphorylation of ERK1/2 in SW480 cancer cells treated with NSC59984 (μmol/L) and NAC (mmol/L) for 16 hours. D, The phosphorylation of ERK1/2 in SW480 cancer cells with knockdown of Ras. SW480 cells were transfected with siRNAs (siRNA #1 and #2) to knockdown Ras, followed with NSC59984 (μmol/L) treatment for 16 hours. E, The phosphorylation of ERK1/2 in SW480 cells with knockdown of Raf expression. C-Raf or B-Raf expression was knocked down by siRNA in SW480 cells, followed with NSC59984 (μmol/L) treatment for 16 hours. F, The phosphorylation of ERK1/2 in SW480 cells treated with NSC59984 (μmol/L) and the different kinase inhibitors (μmol/L) as indicated for 16 hours.