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. 2022 Mar 22;38(5):665–677. doi: 10.1007/s00383-022-05094-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Analysis of dynamic decellularised bladders. Collagen content (A) was unchanged after treatment (p = 0.68); sulfated glycosaminoglycan (GAG) was significantly (*p = 0.006) depleted after decellularisation (B). Mechanical characterization of the acellular matrix: stress–strain curves show the tensile strength and stiffness significantly increased in bladder extracellular matrix (BEM) when compared with fresh tissue (C, D; **p = 0.0113). Pressure–Volume curves are linear both in fresh and decellularised bladders, and showed that BEM were more compliant compared with fresh bladder (E)