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. 2021 Dec 13;12(4):1152–1169. doi: 10.1158/2159-8290.CD-21-0674

Figure 6.

Figure 6. Expression of high FG motif valence NHA9 constructs in lin− HSPCs leads to hematopoietic cell transformation and aberrant expression of Hox family and other genes. A, Average number of colonies per 2,000 cells for lin− HSPCs expressing negative control empty vector and mEGFP-tagged NHA9 and mutant constructs. The values of colony numbers shown are mean ± SD from triplicate technical replicates of a representative experiment. B–D, RNA-seq was performed for lin− HSPCs expressing empty vector, G-NHA9, or mutants after 1 week of growth in methylcellulose containing myeloid and erythroid growth factors (n = 5 for each condition). B, Heat map for differentially expressed genes of interest. C, PCA of the 500 most variable genes. D, Gene set enrichment analysis for cells expressing G-NHA9, G-NHA9–8FA, or G-NHA9Midi—each versus empty vector. Pathways of interest are shown, with a complete list of significantly upregulated or downregulated gene sets in Supplementary Table S3. The most significantly dysregulated genes from each pathway are marked in B.

Expression of high FG motif valence NHA9 constructs in lin− HSPCs leads to hematopoietic cell transformation and aberrant expression of Hox family and other genes. A, Average number of colonies per 2,000 cells for lin− HSPCs expressing negative control empty vector and mEGFP-tagged NHA9 and mutant constructs. The values of colony numbers shown are mean ± SD from triplicate technical replicates of a representative experiment. B–D, RNA-seq was performed for lin− HSPCs expressing empty vector, G-NHA9, or mutants after 1 week of growth in methylcellulose containing myeloid and erythroid growth factors (n = 5 for each condition). B, Heat map for differentially expressed genes of interest. C, PCA of the 500 most variable genes. D, Gene set enrichment analysis for cells expressing G-NHA9, G-NHA9–8FA, or G-NHA9Midi—each versus empty vector. Pathways of interest are shown, with a complete list of significantly upregulated or downregulated gene sets in Supplementary Table S3. The most significantly dysregulated genes from each pathway are marked in B.