TABLE 3.
Pulmonary virus titers 4 days after virus challenge in cotton rats administered EICAR i.p. dailya
| Expt no. and group | Challenge virus | Treatment | Daily dose (mg/kg) | Virus titer (log10/g lung) in cotton rat no.:
|
Mean ± SD titer | |||
|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | |||||
| Expt 1 | ||||||||
| 1 | MV | Placebo | 0 | 4.0 | 4.5 | 4.1 | 3.5 | 4.0 ± 0.4 |
| 2 | MV | EICAR | 50 | 4.0 | 3.5 | 4.0 | 4.0 | 3.9 ± 0.6 |
| 3 | MV | EICAR | 100 | 3.6 | 3.0 | 3.0 | 3.5 | 3.3 ± 0.3 |
| 4 | RSV | Placebo | 0 | 4.0 | 4.5 | 4.1 | 3.6 | 4.1 ± 0.4 |
| 5 | RSV | EICAR | 100 | 3.1 | 2.0 | 2.5 | 3.0 | 2.7 ± 0.5b |
| Expt 2 | ||||||||
| 1 | MV | Placebo | 0 | 4.5 | 3.6 | 4.0 | 4.0 | 4.0 ± 0.4 |
| 2 | MV | EICAR | 120 | 3.5 | 2.0 | 3.0 | 3.0 | 3.0 ± 0.6b |
All of the cotton rats used in these experiments were inoculated intranasally on day 0 with 104 median tissue culture infectious doses of MV or RSV. On each of the next 3 days, the animals were inoculated i.p. b.i.d. with placebo (distilled water) or the indicated dose of EICAR. All of the animals were killed on day 4 after virus inoculation, at which times their lungs were removed and tested for virus.
Boldfaced means and standard deviations indicate statistical significance between means for these groups and the placebo control group (P ≤ 0.05) as determined by the Kruskal-Wallis nonparametric analysis of variance test (experiment 1) or the Mann-Whitney test (experiment 2); the number of animals per group was 4.