Table 3.
Sample identification and processing order.
| Sub-sample order | Sample ID | Experimental condition | Isolate |
|---|---|---|---|
| Round-1 | 1 | Control | D1a |
| 17 | Treatment | D1a | |
| 9 | Control | B1 | |
| 25 | Treatment | B1 | |
| 13 | Control | C1-124 | |
| 29 | Treatment | C1-124 | |
| Round-2 | 10 | Control | B1 |
| 26 | Treatment | B1 | |
| 14 | Control | C1-124 | |
| 30 | Treatment | C1-124 | |
| 2 | Control | D1a | |
| 18 | Treatment | D1a | |
| Round-3 | 11 | Control | B1 |
| 27 | Treatment | B1 | |
| 15 | Control | C1-124 | |
| 31 | Treatment | C1-124 | |
| 3 | Control | D1a | |
| 19 | Treatment | D1a | |
| Round-4 | 16 | Control | C1-124 |
| 32 | Treatment | C1-124 | |
| 4 | Control | D1a | |
| 20 | Treatment | D1a | |
| 12 | Control | B1 | |
| 28 | Treatment | B1 |
A systematic design was used to process samples in four rounds to ensure one replicate of each Symbiodiniaceae isolate and experimental condition. This design was undertaken to account for processing time. The sample ID is used throughout as an identifier, along with the experimental condition (control maintained at ca. 26 °C and treatment maintained at ca. 32 °C) and time points (day 0, (T0), after temperature ramping (day 3, TI), and then at the end of seven days (day 10, TE) (see methods and Table 3).