Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 5;13:1822. doi: 10.1038/s41467-022-29280-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — A two-dimensional representation (top) of a GABA_A receptor β3 subunit depicting binding loops (blue), coupling loops (pink) and four transmembrane regions (orange) is shown. Bar graph summarizing the fold-shift in GABA sensitivity (mean ΔlogEC₅₀ ± s.d.) and maximum current (mean I_max ± s.d.) for each variant according to their amino acid residue. At GABA sensitivity, blue bars indicate variants that have significantly increased the GABA sensitivity with the direction of the bars upwards, red bars indicate variants that have significantly decreased the GABA sensitivity with the direction of the bars downwards and grey bars indicate no significant change. Significance was determined by one-way ANOVA (F (53, 725) = 355.5) and multiple comparisons with corrected Dunnett’s post-hoc test (p < 0.0001, n = 135 independent cells (wild-type); 10 (G32R, M80T, R142L, A305T, A305V, I306T); 11 (E77K, M80K, D120N, T157M, Y182F, Y184H, Q249K, S254F, L256Q, T281I, L284M, T287I, T288N, L293H, P301L); 12 (S76C, V78F, L124F, L170R, E180G, F225C, Y230H, Y245H, P253L, I280F, L284R, L284P, Y302C, F318S, N328D, E357K, S420I, R429Q, S433L); 13 (E178G, T185I); 14 (K127R, L165Q, Q210H, R232Q, T281A); 15 (V37G, N110D, ins138_139H, R166S, I300T); 17 (L310I). At maximum current, grey bars indicate no significant change and red bars indicate significantly reduced maximum currents. Significance was determined by one-way Kruskal–Willis ANOVA (H(55, 1653) = 674.4) and multiple comparisons with corrected Dunn’s post-hoc test (p < 0.0001, n = 256 independent cells (wild-type); 22 (R232P, L284R, I300T, Y302); 23 (D120N, L124F, L284, P301L, A305T); 24 (E77K, V78F, M80K, K127R, L165Q, Q210H, R232Q, Y245H, P253L, S254F, L256Q, I280F, T281A, T281I, L284M, T287I, T288N, L293H, A305V, I306T); 26 (M80T, Y184H, Q249K); 27 (G32R, S76C, R142L, L170R, E180G, Y182F); 28 (L310I); 29 (F318S, E357K, R429Q, S433L); 30 (ins138_139H, Q210H); 33 (V37G), p.(R166S); 36 (S420Ile); 39 (N328D). Individual data points are in the supplementary information (Supplementary Fig S2). Source data are provided in the Source Data file.