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. 2022 Apr 5;12:5664. doi: 10.1038/s41598-022-08422-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Smooth muscle cell phenotypic switching in SM22-Δ mice. (A) Abdominal aorta gene expression from fl/fl (n = 5) and Sm22-Δ (n = 5) mice following 4 week AngII treatment. Data is represented as relative gene expression. Relative gene expression was calculated using an average CT value from fl/fl mice treated with AngII for 4 weeks. fl/fl mice treated with AngII for 4 weeks were used as the control and compared to SM22-Δ mice treated with AngII for 4 weeks. *P < 0.05, unpaired two- tailed student t-test. (B) Immunofluorescence of Acta2 (green) and DAPI (blue) in abdominal aorta from fl/fl and SM22-Δ mice following 28 day AngII treatment. (C) Relative Acta2 fluorescence in abdominal aorta from fl/fl (n = 7) and SM22-Δ (n = 4) mice treated with AngII. Acta2 staining was quantified with Image J and presented as mean ± SEM. *P < 0.05, Welch’s t-test. (D) Representative calponin stained images of abdominal aorta from fl/fl and SM22-Δ mice following 4 weeks AngII treatment. (E) Immunofluorescence of H3K4me2 (red) on Myh11 promoter and Acta2 (green) in abdominal aorta from fl/fl mice following 28 day AngII treatment. Representative image is shown. (F) Number of H3K4me2+ cells per section from fl/fl (n = 7) and SM22-Δ (n = 4) mice following AngII treatment.