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. 2022 Apr 5;12:5664. doi: 10.1038/s41598-022-08422-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Reduced inflammation in SM22-Δ abdominal aorta. (A) Gene microarray were performed in abdominal aorta following 1 week AngII infusion in fl/fl (n = 3) and SM22-Δ (n = 3) mice, *P < 0.05, unpaired two-tailed student t-test. Relative gene expression was calculated using an average CT value from fl/fl mice treated with AngII for 1 week. fl/fl mice treated with AngII for 1 week were used as the control and compared to SM22-Δ mice treated with AngII for 1 week. (B) Abdominal aorta gene expression from fl/fl (n = 4) and SM22-Δ (n = 3) mice following AngII treatment. Data is represented as relative gene expression, *P < 0.05, unpaired two-tailed student t-test. Relative gene expression was calculated using an average CT value from fl/fl mice treated with AngII for 1 week. fl/fl mice treated with AngII for 1 week were used as the control and compared to SM22-Δ mice treated with AngII for 1 week. (C) Gene expression of isolated aortic SMC from fl/fl (n = 3) and SM22-Δ (n = 3) following 24 h AngII treatment. Relative gene expression was calculated using an average CT value from fl/fl mice treated with AngII for 1 week. fl/fl mice treated with AngII for 1 week were used as the control and compared to SM22-Δ mice treated with AngII for 1 week. (D) Representative vimentin stained abdominal aorta from fl/fl andSM22-Δ mice following 1 week AngII infusion and percent area of vimentin stain in abdominal aortas from fl/fl (n = 4) and SM22-Δ (n = 3). Vimentin stain was quantified with Image J and presented as mean ± SEM. *P < 0.05, unpaired two-tailed student t-test. (E) Representative FSP-1 stained abdominal aorta from fl/fl and SM-Δ mice following 1 week AngII infusion. (F) Abdominal aorta Oct4 gene expression from fl/fl (n = 4) and SM22-Δ mice (n = 4) *P < 0.05, unpaired two-tailed student t-test. Relative gene expression was calculated using an average CT value from fl/fl untreated SMCs. fl/fl untreated SMCs were used as the control and compared to untreated SM22-Δ SMCs. (G) Oct4 gene expression of isolated aortic SMC from fl/fl (n = 3) and SM22-Δ (n = 3) following 24 h AngII treatment, *P < 0.05, Two way ANOVA, Holm-Sidak, all pairwise multiple comparison. Relative gene expression was calculated using an average CT value from fl/fl 24 h AngII treated SMCs. fl/fl 24 h AngII treated SMCs were used as the control and compared to 24 h AngII SM22-Δ SMCs.