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. 2022 Apr 5;13:1824. doi: 10.1038/s41467-022-29367-5

Fig. 1. Rapid, short-lived effects of acute stress (AS) on the phosphoproteome of the dorsal hippocampus (dHC) and ventral hippocampus (vHC).

Fig. 1

A Experimental design and tissue collection approach. B Volcano plots showing the estimated log2 fold changes and statistical test results for modified peptides immediately (Exp. 1) or shortly (Exp. 2) after exposure to AS. Red and blue dots represent significant changes within 5% false discovery rate (FDR) relative to non-stressed controls. C Heatmaps showing the abundance of significantly modified peptides across all samples. D Amino acid sequence of Synapsin 1, illustrating overall coverage (grey, green and red), phosphopeptides significantly upregulated (green) or downregulated (red) at any time point after AS and detected phosphosites (blue, probability of post-translational modification >0.75). E Quantification of all detected Synapsin 1 modified peptides spanning sites pS551 and pS553, and their temporal profiles (blue = dHC, red = vHC). F Validation of the upregulation of the phosphorylated site SYN1-pS553 with Western blot in the dHC immediately after stress (Effect size ~300%, t(6) = 2.40, p = 0.037, one-tailed unpaired t test, N = 4 mice/group). Data represent mean ± SEM. Source data are provided as a Source Data file (G) Experimental design of tests for phosphoproteomic sex differences. H Volcano plots showing the estimated log2 fold changes and statistical test results for modified peptides immediately after stress exposure in female mice. Red: significant upregulation (FDR adj. p < 0.05), blue: significant downregulation (FDR adj. p < 0.05) (I) Histogram of interaction p values of all stress or sex responsive phosphopeptides. J AS vs ctrl log2FCs in males (blue) and females (red) sorted by stress response strength in males. Phosphopeptides with significant AS:Sex interaction are labeled in bright red. K Stress responsive phosphopeptides in male and female dHC immediately after stress. *p < 0.05.