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. 2022 Apr 5;13:1824. doi: 10.1038/s41467-022-29367-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Schematic of TRAP. B-D Microscopy images showing co-localization of eGFP with NeuN and GFAP in the hilus and DG of CMV-nuTRAP mice (B), eGFP and mCherry in the ventral hilus, CA3 and DG of CaMKIIa-nuTRAP mice (C), and eGFP with GAD65/67 in the dorsal hilus and DG of vGAT::bacTRAP mice (D). Scale bars 50 μm. Experiments performed 1-2 times with similar results, images representative of <4 mice/line. E–G RT-qPCR validation of TRAP enrichment. Individual values represent dHC and vHC samples from 3–4 mice/line. Data represent mean ± SEM, asterisks indicate p values generated by independent unpaired t-tests. Source data are provided as a Source Data file (E) Genes primarily expressed in excitatory neurons, inhibitory neurons or glial cells are present both before (total RNA) and after (bound RNA) the IP, while the lncRNA AtLAS is reduced in the bound RNA of hippocampal samples from CMV-nuTRAP mice (Nrn1 t(10) = 2.486, p = 0.0322; Gad1 t(10) = 0.2409, p = 0.8145; Gfap t(10) = 0.683, p = 0.5101, AtLAS t(10) = 9.134, p = 3.62e-06). F Depletion of genes not expresse in excitatory neurons, and enrichment of genes primarily expressed in excitatory neurons after IP compared to before, in hippocampal samples from CamKIIa-nuTRAP mice (Gad1 t(5) = 6.65, p = 0.0012; Slc6a1 t(5) = 9.00, p = 0.0003; Gfap t(5) = 3.69, p = 0.0141; Adora1 t(5) = 2.20, p = 0.0794; Neurod6 t(5) = 3.32, p = 0.0210; Nrn1 t(5) = 1.15, p = 0.3012; Slc17a7 t(5) = 2.87, p = 0.0350). G Depletion of genes not expressed in inhibitory neurons and enrichment of genes primarily expressed in inhibitory neurons after IP compared to before, in hippocampal samples from vGAT::bacTRAP mice (Nrn1 t(10) = 10.49, p = 1.03e-06; Slc17a7 t(10) = 11.67, p = 3.80E-07; Gad1 t(10) = 6.23, p = 9.76E-05; Slc6a1 t(10) = 3.01, p = 0.0131). H Correlation between bulk sequencing and CMV-nuTRAP logFCs 45 mins after stress. Colors indicate significance in analyses (red = significant in both datasets, blue = in CMV-nuTRAP only, yellow = in bulk only). I Significant genes that are AS responsive in pooled data from all experiments (time series, CMV-nuTRAP, vGAT::bacTRAP and CaMKIIa-nuTRAP) in the vHC. J Significant genes when employing a model that includes interaction terms in the vHC. K logFC 45 mins after AS across datasets of the top 20 genes in the vHC. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Male CamKIIa-nuTRAP and vGAT::bacTRAP, and female CMV-nuTRAP were used.