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. 2022 Apr 5;8:163. doi: 10.1038/s41420-022-00960-3

Fig. 3. IL-37D transgene promotes WAT homeostasis and beiging via upregulating type 2 cytokines in HFD-fed mice.

Fig. 3

a–d IL-37Dtg and WT mice were fed on 18 weeks of HFD (n = 4 per group). The morphology and weight (a) of inguinal and epidydimal WAT (IngWAT, EpiWAT) are shown. WAT sections were stained with H&E (b) and adipocyte sizes were measured using Image-J (c). The protein levels of UCP-1 (d) were detected by western blot. e–h IL-37Dtg and WT mice fed on 23 weeks of HFD (n = 4 per group). The mRNA levels of IL-6, IL-1β, and TNF-α in WAT explants were determined by qPCR (e). The secretion levels of IL-4 (f) IL-5 (g) and IL-13 (h) from inguinal WAT explants were detected by ELISA. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, determined by two-way ANOVA (a, c) or student’s t test (e–h).