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. 2022 Apr 5;8:18. doi: 10.1038/s41522-022-00283-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Principal coordinates analysis (PCoA) based on the weighted UniFrac distances. Each symbol represents a single sample of the cecal content after 48 h of cold exposure (n = 5) or RT controls (n = 5 per group). b Heatmap tree comparing the most abundant ASVs from the cecum content of 48 h cold-exposed animals (n = 5, inner green rings) and RT controls (n = 5, outer pink rings) and their phylogenetic relationships. The bar represents abundant ASVs. Firmicutes and Bacteroidetes appear in orange and blue colors, respectively. c The alpha diversity of ASVs. d–i Boxplot of the microbiota of the cecal contents at the phylum level. j The different ASVs with a bar and heatmap with P < 0.05. The data are the means ± SEMs (n = 5 per group).