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. 2022 Apr 5;13(4):305. doi: 10.1038/s41419-022-04562-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Senescence (p16 and p21) and SASP markers (IL-6, MCP-1, Pai1) in different tissues detected by qRT-PCR after a four-month treatment. n = 5. Data are presented as means ± SD. Differences were assessed by the one-way ANOVA test. *P < 0.05, **P < 0.01. **P < 0.001, ****P < 0.0001. B Main SASP factor in the serum was detected by CBA after a four-month treatment. n = 5. Data are presented as means ± SD. Differences were assessed by the one-way ANOVA test. *P < 0.05, **P < 0.01, **P < 0.001, ****P < 0.0001. C NAD levels in fat and liver. n = 5. Data are presented as means ± SD. Differences were assessed by the one-way ANOVA test after a four-month treatment. *P < 0.05, **P < 0.01. **P < 0.001. D The levels of ALT, AST, CREA, UA, CHOl, and BUN in aged mice after a four-month treatment. n = 5. Data are presented as means ± SD. Differences were assessed by the one-way ANOVA test. ns P > 0.05, **P < 0.01. Two parallel samples were set in each experiment and three independent experiments were performed for each result.