Fig. 2. Transplantation of adipose tissue derived from Lepr^db/db mice into Lepr^db/+mice impairs wound healing.

a Schematic diagram of the procedure for the adipose tissue transplantation (ATT) experiments. b Representative image of H&E staining and MT staining of a wound at 8 DPW and (c) the quantitative analysis of the wound healing histological score (n = 8 in the ATT group and n = 4 in the sham group). d, e Representative images of PDGFRα and α-SMA immunostaining of a wound edge at 8 DPW and quantitative data on the percentage of PDGFRα and α-SMA + cells in interstitial connective tissue (n = 4 for each group). f Relative levels of Acta2 and Pdgfra mRNA in Lepr^db/+ and Lepr^db/db mouse skin at 8 DPW. g, h Localization of CM-DiI–labeled adipose tissue–derived cells within wound regions and quantitative data (n = 3 for each group). Quantitative data are shown as means ± SE in either dot plots or box-and-whisker plots with IQRs and 1.5 times the IQR. p-values were determined using a two-tailed Student’s t-test or the Tukey method for one-way ANOVA (*p < 0.05 and **p < 0.001).