Fig. 6. Components of post-wound adipose tissue-derived cytokines in Lepr^db/+and Lepr^dbdb+ mice and their effect on cell migration.

a Proteome profiler antibody array analysis of SASP-containing culture media (CM) collected from the organ culture of adipose tissue at pre-wounding, 2 DPW, and 8 DPW. b Hierarchical clustering of differentially expressed cytokine expression. Cytokines with higher expression are depicted in red, cytokines with lower expression are depicted in blue, and cytokines with no difference are depicted in white. c Representative phase-contrast image of wound assay treated with adipose tissue-derived cultured media collected from either Lepr^db/+ or Lepr^db/db mice at 2DPW and (d) the percentage of wound closure at each time point, from immediately after wounding (D0) to 4 d after wounding (D4) (Ctrl DMEM: n = 15; Lepr^db/+ CM: n = 12; Lepr^db/+ CM: n = 15). Quantitative data are shown as box-and-whisker plots with IQRs and 1.5 times the IQR. p-values were determined using the Tukey method for one-way ANOVA (*p < 0.05 and **p < 0.001).