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. 2022 Apr 5;5:312. doi: 10.1038/s42003-022-03269-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Ribbon representation of the structure of the human FAM92A-Chibby1 complex as predicted by AF2. The top five predictions were virtually identical in the FAM92A BAR domain and the Chibby1 beta-sheet domain and mainly differed in the orientation of Chibby1’s coiled-coil domain. The different coiled-coil orientations are shown for all five models (in blue or grey). Shown boxed is a detailed view on the interface between the beta-sheet domain of Chibby1 and the BAR domain of FAM92A. Selected sidechains are shown as sticks and are labelled. Dotted yellow lines designate hydrogen bonds. The rotation angles to obtain this view are indicated. In the domain organisation scheme of FAM92A and Chibby1, cc denotes a predicted coiled coil and amino acid residue numbers indicate domain boundaries. Coloured in red are the protein parts shown in the structural models. b The AF2-predicted interface between the Chibby1 beta-sheet domain and the FAM92A BAR domain is essential for complex formation. Western blot showing a pull-down experiment with lysates from cells expressing 3xHA-tagged FAM92A or the indicated 3xFLAG-tagged Chibby1 constructs. TCL: Total cell lysate. c As in panel b, but pulldown with 3xFLAG-tagged Chibby1 and the indicated 3xHA-tagged FAM92A constructs.