Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 5;13:1819. doi: 10.1038/s41467-022-29485-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Left, activity assay of USPL1 with SUMO1-AMC and SUMO2-AMC. Middle, activity assay of Ubiquitin-AMC with USPL1 and USP28. Right, activity assay of SENP2 with SUMO1-AMC and SUMO2-AMC. Reactions were conducted in triplicate and the average curve is displayed. b Binding interaction of USPL1 point mutants with SUMO2-DHA. Reaction assays were conducted with USPL1 wild-type and mutants for 3 h. n = 3 technical replicates. c USPL1-SUMO2 complex structure shown in cartoon representation. USPL1 catalytic domain and SUMO2 precursor are shown in purple and red, respectively. USP right hand-like domains are labeled. Interface residues are labeled and shown in stick representation. Zinc atom is shown in yellow. d Left, activity assays of USPL1 wild type and mutants of the C-terminal tail interface using SUMO2-AMC. Middle, similar activity assays of USPL1 wild type and mutants of the thumb and fingers subdomains. Right, table indicating the mean slope values plus/minus the standard deviation of the activity assays. n = 3 technical replicates. Significance was measured by a two-tailed unpaired t-test relative to wild-type. *P < 0.05, **P < 0.01, ***P < 0.001. e Left, endpoint assays of USPL1 wild type and mutants using the RanGAP1-SUMO2 substrate. Right, plot of the RanGAP1-SUMO2 fraction after 10 min reaction. Data values represent the mean ± SD, n = 3 technical replicates. Significance was measured by a two-tailed unpaired t-test relative to wild-type. *P < 0.05, **P < 0.01, ***P < 0.001. Exact P values from left to right: <0.0001, 0.0868, <0.0001, 0.0002, <0.0001, 0.0001, 0.0065, <0.0001, <0.0001, <0.0001. f Left, endpoint assays of USPL1 wild type and mutants using di-SUMO2 substrate. Right, plot representation of the product SUMO2 fraction after 20 min reaction. Data values represent the mean ± SD, n = 3 technical replicates. Significance was measured by a two-tailed unpaired t-test relative to wild-type. *P < 0.05, **P < 0.01, ***P < 0.001. Exact P values from left to right: <0.0001, <0.0001, 0.0003, <0.0001, 0.0021, <0.0001, 0.0009, <0.0001, <0.0001, <0.0001. Source data for Fig. 6a, b, d, e, and f are provided as a Source Data file.