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. 2022 Apr 5;13:1841. doi: 10.1038/s41467-022-29421-2

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© The Author(s) 2022, corrected publication 2022

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Fig. 8 — a Flow cytometry of germinal centre B cells (CD19⁺CD38^lo/-GL7⁺) (left) and dark (CD19⁺CD38^lo/-GL7⁺CXCR4^hiCD86^lo) and light zone (CD19⁺CD38^lo/-GL7⁺CXCR4^loCD86^hi) (right). The percentage of germinal centre B, dark zone and light zone at day 14 after immunization are represented. Gating strategies are presented in Supplementary Fig. 27. b The percentage of cells in the germinal centre B cells in CD19⁺ B cells of ICR (green) and TC-mAb (blue) mice. All gating panels of germinal centre B cell subsets are presented in Supplementary Fig. 28. P-values (two-tailed unpaired Student’s t test) between ICR and TC-mAb mice of the percentages of germinal centre B subsets were 0.202 (Pre, pre-immune), 0.139 (day 7), 0.790 (day 14), 0.002 (day 21) and 0.013 (day 28). P-values between pre and a peak timepoint of germinal centre B subset percentages were 0.236 (ICR, pre and day 14) and 0.009 (TC-mAb, pre and day 21). c The ratio of dark and right zone. All gating panels of germinal centre B cell subsets are presented in Supplementary Fig. 29. P-values between ICR and TC-mAb mice of the ratio of dark and light zone B subsets were 0.097 (Pre), 0.006 (day 7), 0.345 (day 14), 0.061 (day 21) and 0.626 (day b 28). d Phosphorylation status of Syk of B cells. e B cells from ICR and TC-mAb mice were cultured in the absence (grey) or presence (sky blue) of BAFF for 24, 48 or 72 h. Staining with TOPRO3 was analysed by flow cytometry and percentages of TOPRO3-negative gated cells (live cells) are shown. Data are presented as means. Error bars indicate the standard deviation of triplicate measurements. Results were pooled from two independent experiments. *P < 0.05 (two-tailed unpaired Student’s t test).