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. 2022 Apr 5;8:164. doi: 10.1038/s41420-022-00925-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A The target binding sites of SNHG12 and miR-129-5p predicted by the RNA22 website. B The target binding sites of E2F7 and miR-129-5p predicted by the starbase website. C The expression box plot of E2F7 in STAD, the gray box represents the expression in normal samples, and the red box the expression in GC samples. D Interaction between SNHG12 and E2F7 in HPMCs detected using RIP assay. E WT and MUT binding sites between SNHG12 and miR-129-5p as well as between miR-129-5p and E2F7. F Luciferase activity analyzed using dual-luciferase reporter gene assay after HEK-293T cells were co-transfected with WT or MUT of SNHG12 or E2F7, miR-129-5p mimic or NC. G The co-localization of Cy3-miR-129-5p (red) and FITC-SNHG12 (green) in HPMCs cells measured using FISH (scale bar = 25 μm), and the nucleus was stained with DAPI (blue). H Direct binding between SNHG12 or E2F7 and miR-129-5p in HPMCs detected by RNA pull-down assay. I miR-129-5p expression pattern in HPMCs evaluated by RT-qPCR. J E2F7 protein expression pattern in HPMCs measured by western blot analysis. p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The experiment was conducted three times independently.