Table 3.
Main features of the two long-read sequencing platforms: PacBio CCS and ONT.
| Features | PacBio CCS | Oxford Nanopore Technologies (ONT) |
|---|---|---|
| Read lenght | > 10 Kbp | Up to 1Mbp |
| Pitfalls | High costs | Relatively high raw read error rates (7%, but reduced to 1.1% when applying isONcorrect); but lower costs |
| *Estimated costs per starter pack | ~ $350,000 (PacBio Sequel) | Variable from $1,000 (MinION), to $50,000 (GridION) or $200,000 (PromethION) |
| Accuracy | > 99% | 98% |
| Estimate of intraplatform reproducibility | Good | Unknown |
| Limitations | Low coverage of masked satellite regions | |