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. 2022 Mar 23;13:867358. doi: 10.3389/fphys.2022.867358

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Copyright © 2022 Shimura and Shaw.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GJA1-20k increases interaction between ER and mitochondria. (A) Representative fixed-cell images of ER and mitochondria in GFP-tagged GJA1-20k (or GST as control) transfected HeLa cells. ER and Mitochondria are visualized by PDI and Tom20 antibodies, respectively. Scale bars, 10 μm. (B) The quantified Pearson’s correlation between ER and Mitochondria with or without exogenous GJA1-20k. n = 12 (GST) or 14 (GJA1-20k) cells. Graphs are expressed as mean ± SD. p value is determined by two-tailed Mann-Whitney U-test. ***p < 0.001. **These figures are published in Reference (Shimura et al., 2021b) as a response to Reviewer’s comments for the corresponding eLife publication (Shimura et al., 2021a).