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. 2022 Mar 23;13:847905. doi: 10.3389/fphar.2022.847905

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Copyright © 2022 Bai, Chen, Hu, Wang, Wu and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silibinin induced apoptosis in HuCCT-1 and CCLP-1 cells. (A,B). Detection of apoptosis by FCM and quantitative analysis of the apoptosis rate. HuCCT-1 and CCLP-1 cells were treated with IC50 and 2*IC50 concentrations of silibinin for 48 h (C). The live/dead staining assay results of the HuCCT-1 and CCLP-1 cell lines treated with silibinin for 48 h. Red dots represent dead cells and green dots represent surviving cells. (D). Western blotting results of HuCCT-1 and CCLP-1 cell lines treated with IC50 and 2*IC50 concentrations of silibinin for 48 h. The apoptosis-related proteins were identified. (E). The quantification results of cle-caspase-9, cle-caspase-3 and cle-PARP. Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, significantly difference compared to the control group.