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. 2022 Mar 23;13:809157. doi: 10.3389/fphar.2022.809157

FIGURE 4.

FIGURE 4

PA aggravated cellular senescence of endothelial cells in Kawasaki disease. (A,B) HUVECs were co-treated with 500 μM PA and 10% KD serum (line 4), or only PA (line 2), or only KD (line 3). Control groups were co-treated with 1%BSA and 10%FBS (line 1). Incubated for 24 h, total proteins were probed for P16 and pRB proteins; Relative protein expression levels of P16 and pRB compared with GAPDH; (C,D) HUVECs were treated with 500 μM PA or 10%KD serum or both compared with control group. The staining of SA-β-gal activity indicated PA aggravated cell senescence in HUVECs (C); Quantification of SA-β-gal staining (D); (E,F) HUVECs were treated with 500 μM PA or 10%KD serum or both, and compared with the control group. Endothelial cell function was detected by cell migration (E); the bar graph represented the quantification of the scratch assay (F). (n = 6; Data shown as Mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).