Figure 4.

Antimicrobial activities of Tatritin against S. aureus, S. agalactiae, A. hydrophila, K. pneumoniae, ESBLs-EC, MRSA, and C. albicans. Antibacterial activity of Tatritin peptide against Gram-positive, -negative bacteria (A) and drug-resistant bacteria (B). The bacteria in the mid-logarithmic phase were diluted in medium and incubated with serially diluted peptide at a concentration of 10⁵ CFU/ml for 18 h. OD₅₉₅ was measured and corrected by initial OD₅₉₅ values. Bacterial growth rates were calculated as the bacterial density in the presence of peptide to the bacterial density of controls. Data shown are the means of duplicate reactions in a representative of three independent experiments. (C) Antifungal activity of Tatritin peptide. Approximately 2 × 10⁵ cells of C. albicans were mixed with Tatritin peptide diluted serially to 1, 2, 4, 8, and 16 μM. The minimum concentration in petri dish without colonies was regarded as the minimum fungicidal concentration. (D) The killing kinetics of Tatritin against Gram-positive and -negative bacteria. Bacteria were treated with Tatritin peptide (20 μM). The viability of bacteria was measured at indicated time points. Samples were measured in triplicates.