Figure 7.

Protective efficacy of Tatritin in the zebrafish model of infection. (A) Schematic of the A. hydrophila-infected prophylactic and therapeutic model. (B) Preventive efficacy of Tatritin in the A. hydrophila-infected prophylactic and therapeutic model. Zebrafish were received intraperitoneal injection at a single dose of 2 µg/g Tatritin or equal volumes of PBS (n = 20) at 24 h before or 3 h after the pathogenic bacteria of A. hydrophila was injected with a lethal dose of 5.6 × 10⁴ CFU. The mortality in each group was recorded for at least 7 days. Experiments were conducted in triplicates, and the data shown are the means. Asterisk indicates that the difference is significant. (C) At 3 days postinfection, bacterial loads in gut and liver of zebrafish infected with A. hydrophila in prophylaxis and therapy groups were detected, (n = 3). *P<0.05, NS, not significant.