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. 2022 Mar 23;13:794779. doi: 10.3389/fimmu.2022.794779

Table 1.

Primers used for amplification of Tatritin and qRT-PCR in this study.

Names Sequences (5′~3′) Description Tm (˚C)
Tatritin_ORF F: CCAGTGACTTAGCGGTACG
R: AGCCTCAAGAAGTATGTGATGA
Amplification of Tatritin ORF 58~60
Tatritin_3’end F1: GGCAGAGCACAAGTTTCC
F2: GCGATGCTTACGCTGGTTGTTTTGA
3′ end specific upstream primers 56~58
Tatritin_qRT-PCR F: GTTACACCTTCCGCCATAT
R: GTTGCCGCTTATCTTGTTAA
Expression analysis of Tatritin 58~62
Gapdh F: ATCATCAGCAATGCCTCTTG
R: GCCTTAGAGCTTGGTCCATC
Reference gene 58~62