Table 1.
Primers used for amplification of Tatritin and qRT-PCR in this study.
| Names | Sequences (5′~3′) | Description | Tm (˚C) |
|---|---|---|---|
| Tatritin_ORF | F: CCAGTGACTTAGCGGTACG R: AGCCTCAAGAAGTATGTGATGA |
Amplification of Tatritin ORF | 58~60 |
| Tatritin_3’end | F1: GGCAGAGCACAAGTTTCC F2: GCGATGCTTACGCTGGTTGTTTTGA |
3′ end specific upstream primers | 56~58 |
| Tatritin_qRT-PCR | F: GTTACACCTTCCGCCATAT R: GTTGCCGCTTATCTTGTTAA |
Expression analysis of Tatritin | 58~62 |
| Gapdh | F: ATCATCAGCAATGCCTCTTG R: GCCTTAGAGCTTGGTCCATC |
Reference gene | 58~62 |