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. 2022 Mar 23;13:810157. doi: 10.3389/fgene.2022.810157

FIGURE 4.

FIGURE 4

Mxi1-0 but not Mxi1-1 promotes PASMCs growth under hypoxic conditions. (A) PASMCs were transfected with siRNAs targeting either Mxi1-0 or Mxi1-1, and were subjected to Western blotting analyses. (B) PASMCs cultured in normoxic or hypoxic conditions were transfected with siRNAs targeting either Mxi1-0 or Mxi1-1, and CCK-8 assays were performed on indicated times. (C) PASMCs cultured in normoxic or hypoxic conditions were infected with recombinant lentiviruses to overexpress Mxi1-0 or Mxi1-1, and CCK-8 assays were performed on indicated times. (D) PASMCs were transfected with Mxi1-0-targeted siRNAs or infected with recombinant lentiviruses to overexpress Mxi1-0, and were cultured in normoxic or hypoxic conditions for 48 h. Cells were then subjected to immunofluorescence staining for EdU, followed by microscopy and calculation of the ratios of EdU-positive cells in three random fields. Scale bar, 100 μm. Data from three independent experiments are shown as means ± SDs. For statistical significance, *represents p < 0.05 compared to siControl, **represents p < 0.01 compared to siControl, ***represents p < 0.001 compared to siControl, ## represents p < 0.01 compared to Hypoxia-siControl, ### represents p < 0.001 compared to Hypoxia-siControl, $represents p < 0.05 compared to Hypoxia-OEmock, ¥represents p < 0.05 compared to Normoxia-OEmock.