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. 2022 Mar 23;13:810157. doi: 10.3389/fgene.2022.810157

FIGURE 6.

FIGURE 6

Mxi1-0 upregulates c-Myc through MEK/ERK signaling in hypoxic PASMCs. (A,B) PASMCs tranfected with siRNAs targeting Mxi1-0 (A) or Mxi1-1 (B) were cultured under normoxic or hypoxic conditions for 48 h. Western blotting assay was performed with the indicated antibodies. All the phospho-protein levels were measured by densitometry and normalized to that of β-actin. (C) PASMCs were cultured under normoxic or hypoxic conditions for 48 h and treated with different doses of PD98059, and the levels of indicated proteins were determined by Western blotting analysis and quantified by densitometry. (D) PASMCs were cultured under normoxic or hypoxic conditions, treated with PD98059 or 10058F4, and infected with control or Mxi1-0-overexpressing lentiviruses. Cells were then subjected to CCK-8 assays on indicated times. Data from three independent experiments are shown as means ± SDs. For statistical significance, *represents p < 0.05 compared between two groups or to Normoxia (D), **represents p < 0.01 compared between two groups or to Normoxia (D), ¥represents p < 0.05 compared between Hypoxia and Hypoxia + PD98059 (40 μM), ¥¥represents p < 0.01 compared between Hypoxia and Hypoxia + PD98059 (40 μM),¥¥¥represents p < 0.001 compared between Hypoxia and Hypoxia + PD98059 (40 μM), ## represents p < 0.01 compared between Hypoxia and Hypoxia+10058F4 (40 μM), ###represents p < 0.001 compared between Hypoxia and Hypoxia + 10058F4 (40 μM).