FIGURE 5.

Effects of EMPA on the AMPK/SIRT1 pathway and oxidative stress in AT1-AA-induced PE mouse kidneys. (A) Protein levels of SIRT1, AMPK, p-AMPK, Foxo1 and SOD2 in the kidney determined by Western blot analysis. (B) Densitometric evaluation of Western blots for SIRT1 (n = 3), (C) p-AMPK (n = 3), (D) Foxo1 (n = 3) and (E) SOD2 (n = 3). (F) Representative immunofluorescence images of SIRT1, p-AMPK, Foxo1 and SOD2 in kidneys of different groups. Scale bar: 50 μm. Data are expressed as the mean ± SEM and were analyzed by ANOVA with LSD post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001.