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. 2022 Mar 23;13:826792. doi: 10.3389/fphar.2022.826792

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Copyright © 2022 Zhai, Liu, Tong, Yu, Yang, Gu and Niu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of EMPA on fetal outcomes in PE mice. (A) Histogram showing the number of live pups in each group (n = 6). (B) Representative images showing the gross morphology of placentae (top) and embryos (bottom) in each group. (C) Histogram showing the fetal weight in each group (n = 6). (D) Whole-mount alcian blue and alizarin red staining of 1-week-old offspring in each group. (E) Pup weights recorded from 0 to 10 weeks of each group (n = 8). (F) Serum BUN levels of each group of offspring at 10 weeks of age (n = 6). (G) H&E staining of hearts, livers, spleens, lungs and kidneys of offspring mice in each group. Scale bar: 200 μm. The results are expressed as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.