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. 2022 Mar 23;13:826792. doi: 10.3389/fphar.2022.826792

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Copyright © 2022 Zhai, Liu, Tong, Yu, Yang, Gu and Niu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EMPA treatment during pregnancy limited the postpartum renal injury response to ADR in AT1-AA-injected maternal mice. (A) SDS–PAGE Coomassie blue staining of bovine serum albumin (BSA) standards and urine from postpartum mice before and after ADR injection, demonstrating proteinuria in these mice; 16 μL of each urine sample was loaded. (B) Quantification of urinary ACRs of postpartum mice before (n = 9) and after ADR (n = 6) injection. (C) Serum BUN levels of PE mice before (n = 3) and after ADR (n = 6) injection. (D) Quantification of the percentage of sclerotic glomeruli in PE mice 4 weeks after ADR injection (n = 3). (E) Representative H&E staining and (F) PAS staining of kidney sections in postpartum mice before and after ADR injection (scale bar represents 50 μm). Data are expressed as the mean ± SEM and were analyzed by ANOVA with LSD post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001.