FIGURE 1.

(A) Cell viability in control, shNC, and shATP7B transfected after 24-h incubation with different concentrations CuSO₄ ( $\overline{x}\pm s,n=6$ ). ^# p < 0.05, ^## p < 0.01 compared with corresponding blank group without CuSO₄ treatment; ^* p < 0.05, ^** p < 0.01 compared between shNC and shATP7B groups at same copper concentration; (B) dose and time-effect curves of shATP7B transfected cell viability after incubation with CuSO₄ ( $\overline{x}\pm s,n=6$ ); (C) cell viability after 24 h treated by GDD ranging from 0 to 320 μg/ml (0, 10, 20, 40, 80, 160, and 320 μg/ml) ( $\overline{x}\pm s,n=6$ ). ^* p < 0.05, ^** p < 0.01 compared with control group; (D) cell viability in copper-laden HLD hepatocytes before and after GDD treatment ( $\overline{x}\pm s,n=6$ ). ^* p < 0.05, ^** p < 0.01 compared with shATP7B + Cu group.