Figure 2.

Yeast cells with Inh1-GFP start budding faster than the cells without Inh1-GFP. Post-diauxic phase yeast cells expressing Inh1-GFP were transferred into a fresh yeast peptone dextrose (YPD) medium. We observed two types of cells: those expressing Inh1-GFP and those with no detectable GFP signal. We calculated the percentages of budded vs. unbudded cells separately for these groups. (A) Representative photograph of the cells at the beginning of the experiment and after 4 h of growth in the fresh medium. DIC—Differential Interference Contrast; (B) Change in the proportion of (1) Inh1-GFP positive non-budding; (2) Inh1-GFP negative non-budding; (3) Inh1-GFP positive budding; and (4) Inh1-GFP negative budding cells. The average proportions are shown as black lines, the results of the individual experiments are illustrated with semi-transparent green or red areas (data of five separate experiments, 369 cells on average in each data point). The number of budding cells with GFP (category 3) increased significantly more than the number of budding cells without GFP (category 4); p = 0.016 according to Wilcoxon rank sum test (n = 5).