FIG. 3.

Schematic representation of the competition assay for compounds that bind to the transglycosylase sites of PBPs. A membrane extract of a PBP 4, PBP 5, and PBP 6 deletion mutant was used for the example shown here. The arrows indicate PBPs that are bound to a soluble moenomycin antagonist (X) and that are therefore washed out. Thereby, the amount of PBPs retained by the moenomycin beads is decreased.