Table 1.
Supporting protein identification evidence for example differently expressed proteins from Figure 1 , the pDC secretome: baseline vs influenza induced*.
| UniProt ID | Protein | Mascot Peptide Count | PD # Unique Peptides | Mascot Protein Score | Mascot Ion Score | Mascot Homology Threshold | PD % Coverage | PD # PSM | Ratio (Flu stim/NO STIM) | P-value (Flu stim vs. NO STIM) |
|---|---|---|---|---|---|---|---|---|---|---|
| CATC_HUMAN | Dipeptidyl peptidase 1 | 14 | 15 | 719 | 62 | 16 | 41.3 | 22 | 33.0 | 6.3E-06 |
| COR1B_HUMAN | Coronin-1B | 5 | 3 | 144 | 45 | 17 | 6.2 | 3 | 0.4 | 3.4E-12 |
| CXCL9_HUMAN | C-X-C motif chemokine 9 | 8 | 5 | 304 | 56 | 17 | 42.1 | 7 | 147.1 | 2.8E-04 |
| CXL10_HUMAN | C-X-C motif chemokine 10 | 6 | 6 | 318 | 56 | 17 | 47.6 | 10 | 117.7 | 4.7E-07 |
| DPYL2_HUMAN | Dihydropyrimidinase-related protein 2 | 21 | 21 | 1065 | 61 | 17 | 55.4 | 25 | 0.5 | 3.4E-27 |
| FSCN1_HUMAN | Fascin | 12 | 10 | 405 | 47 | 17 | 25.6 | 12 | 11.0 | 6.1E-07 |
| GRAB_HUMAN | Granzyme B | 2 | 6 | 107 | 41 | 16 | 20.0 | 6 | 18.1 | 3.4E-04 |
| H2AY_HUMAN | Core histone macro-H2A.1 | 12 | 10 | 369 | 56 | 18 | 37.2 | 12 | 0.4 | 2.6E-13 |
| IFIT3_HUMAN | Interferon-induced protein with tetratricopeptide repeats 3 | 7 | 6 | 249 | 45 | 16 | 19.7 | 8 | 40.5 | 8.1E-09 |
| IFN10_HUMAN | Interferon alpha-10 | 2 | 3 | 427 | 60 | 16 | 27.7 | 22 | 306.2 | 0 |
| IFN14_HUMAN | Interferon alpha-14 | 4 | 4 | 837 | 46 | 16 | 46.6 | 42 | 508.0 | 1.0E-33 |
| IFN16_HUMAN | Interferon alpha-16 | 6 | 5 | 789 | 61 | 17 | 46.6 | 24 | 94.9 | 3.1E-28 |
| IFN17_HUMAN | Interferon alpha-17 | 2 | 2 | 696 | 68 | 17 | 47.4 | 33 | 91.6 | 0 |
| IFN21_HUMAN | Interferon alpha-21 | 7 | 1 | 1432 | 64 | 17 | 52.6 | 59 | 468.2 | 2.0E-21 |
| IFNA1_HUMAN | Interferon alpha-1/13 | 10 | 10 | 947 | 52 | 18 | 51.5 | 47 | 499.3 | 4.6E-16 |
| IFNA2_HUMAN | Interferon alpha-2 | 4 | 4 | 533 | 59 | 18 | 36.2 | 20 | 296.3 | 2.2E-18 |
| IFNA4_HUMAN | Interferon alpha-4 | 4 | 2 | 607 | 50 | 16 | 36.0 | 31 | 549.7 | 2.3E-30 |
| IFNA5_HUMAN | Interferon alpha-5 | 2 | 2 | 1333 | 56 | 21 | 46.6 | 58 | 56.1 | 1.3E-25 |
| IFNA6_HUMAN | Interferon alpha-6 | 5 | 5 | 361 | 28 | 16 | 38.1 | 26 | 233.7 | 1.1E-28 |
| IFNA7_HUMAN | Interferon alpha-7 | 10 | 3 | 390 | 35 | 15 | 45.5 | 27 | 469.5 | 0 |
| IFNA8_HUMAN | Interferon alpha-8 | 4 | 7 | 947 | 41 | 17 | 46.9 | 48 | 901.5 | 0 |
| IFNB_HUMAN | Interferon beta | 3 | 7 | 93 | 33 | 17 | 27.8 | 7 | 647.0 | 3.6E-40 |
| IFNL1_HUMAN | Interferon lambda-1 | 3 | 4 | 151 | 34 | 18 | 26.8 | 6 | 31.7 | 3.5E-05 |
| IFNL3_HUMAN | Interferon lambda-3 | 2 | 3 | 222 | 42 | 18 | 36.4 | 6 | 186.3 | 2.0E-23 |
| IFNW1_HUMAN | Interferon omega-1 | 3 | 8 | 182 | 30 | 16 | 26.2 | 16 | 44.2 | 0 |
| ISG15_HUMAN | Ubiquitin-like protein ISG15 | 5 | 4 | 218 | 49 | 17 | 26.9 | 5 | 24.5 | 2.0E-03 |
| LGMN_HUMAN | Legumain | 4 | 3 | 135 | 56 | 17 | 13.4 | 3 | 11.0 | 1.8E-10 |
| PNCB_HUMAN | Nicotinate phosphoribosyltransferase | 4 | 3 | 89 | 35 | 17 | 8.1 | 3 | 0.4 | 9.5E-25 |
| SQSTM_HUMAN | Sequestosome-1 | 1 | 3 | 74 | 50 | 16 | 11.0 | 3 | 46.0 | 4.0E-03 |
| TNFA_HUMAN | Tumor necrosis factor | 5 | 6 | 137 | 38 | 16 | 37.8 | 8 | 72.1 | 3.5E-17 |
*Data generated by Mascot server V. 2.5.1., Proteome Discoverer v. 1.4 (for protein identification), or Elucidator V. 4.0.0.2.31 (for differential expression).