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. 2022 Apr 6;12(4):210282. doi: 10.1098/rsob.210282

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© 2022 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 6. — Microexamination of mycelium development of the wild-type strain and ΔPcDHCR7 transformants and green fluorescence localization in eGFP labelled transformants. (a) Morphology of different strains after 1 day's germination of cystospores. WT indicates the wild-type strain BYA5; KD1-1 and KD2-1 are representative ΔPcDHCR7 transformants; ReD1-1 is a PcDHCR7-complemented transformant. (b,c) Typical green fluorescence localization of a representative transformant expressing PcDHCR7-eGFP (b) or eGFP protein (c). Bright field (left), GFP field (middle) and merged field (right) are shown. Bar, 50 µm.