TABLE 1.

Kinetic parameters for binding of efavirenz, sefavirenz, and nevirapine to different catalytic forms of HIV-1 RT

Drug	Catalytic form	Mean ± SD
		Kd (nM)	kapp (s−1)	kon (s−1 M−1)a	koff (s−1)b
Efavirenz	RT	170 ± 5	NAc	NA	NA
	RT-TPd	30 ± 2	(6 ± 1) × 10−3	(1.3 ± 0.2) × 104	(4 ± 1) × 10−4
	RT-TP-dNTPe	4 ± 0.5	(7 ± 1) × 10−3	(3.4 ± 0.2) × 104	(1.6 ± 0.5 × 10−4
Sefavirenz	RT	750 ± 15	NA	NA	NA
	RT-TPd	230 ± 20	(4 ± 1) × 10−3	(0.5 ± 0.1) × 104	(1 ± 0.5) × 10−3
	RT-TP-dNTPe	8 ± 0.5	(6 ± 1) × 10−3	(5.5 ± 0.5) × 104	(4 ± 1) × 10−4
Nevirapine	RT	400 ± 15	NA	NA	NA
	RT-TPd	400 ± 20	(5.7 ± 1) × 10−3	(0.15 ± 0.03) × 104	(6 ± 0.5) × 10−4
	RT-TP-dNTPe	450 ± 25	(6 ± 1) × 10−3	(0.2 ± 0.05) × 104	(6 ± 1) × 10−4

^aCalculated from k_app = k_on (K_i + [I]). 

^bCalculated from K_d = k_off/k_on. 

^cNA, not applicable.