FIGURE 1.

MIK2 contributes to Arabidopsis immunity against a generalist herbivore. (A) Insect performance of Spodoptera littoralis on Col-0 and mik2-1. S. littoralis larvae were feeding on 5-week-old plants for 12 days. Means ± SEM of three independent biological replicates are shown. Asterisks denote statistical differences between larvae feeding Col-0 and mik2-1: ***P < 0.001 (Mann–Whitney U test). Symbols indicate individual values and symbol shapes (circle, square, and triangle) indicate different biological replicates. (B,C) Jasmonate levels in Col-0 and mik2-1. Absolute levels of total JA (B) and JA-Ile (C) were measured by UHPLC-MS/MS after 2 days of S. littoralis feeding. Non-infested plants served as controls. Values represent means ± SEM of three independent biological replicates. Letters denote statistical differences (ANOVA followed by Tukey’s HSD). Different symbols indicate different biological replicates.