Table 1.

Comparison of methods used for complete inactivation of SARS-CoV-2 in clinical samples.

	Type	Stage	Protocol/Reagent used		Reference
Virus inactivation	Heat treatment	Before direct analysis, or before RNA extraction	Lower temperature, longer duration	Temperature above 80°C, during at least 1 hour;	(Burton et al., 2021)
			Higher temperature, brief duration	Temperatures above 90°C can inactivate samples in a few minutes;	(Burton et al., 2021)
	Chemical methods	Transport	VTM	Primestore MTM, 4M GITC/Tx TM, COPAN eNAT;	(Welch et al., 2020; Richard-Greenblatt et al., 2021)
		Before analysis without full RNA extraction, or in the process of full RNA extraction	Non-anionic detergents	Triton X-100;	(Welch et al., 2020)
			Lysis buffer	ATL, VXL, AVL, Phanter fusion specimen lysis tubes, MagNA Pure External LB, RLT, E&O Lab LB;	(Pastorino et al., 2020b; Welch et al., 2020)
			Extraction reagents	Trizol, Trizol LS;	(Patterson et al., 2020)
		Before direct analysis,	Other	TCEP+ EDTA + Heat.	(Rabe and Cepko, 2020)