Table 3.

Compilation of strategies of non-isothermal amplification explored for SARS-CoV-2 diagnostic.

	Method		Method´variation	Region targeted	Single or multiple targeting	Duration of amplification (min)	Included in assay issued with EUA	Source
Non-isothermal amplification	PCR-based	RT- qPCR	Single target RT-qPCR	ORF1ab, RdRp, N, E, S	Single	50	Yes	(Jung et al., 2020; Park et al., 2020)
			Multiplex RT-qPCR	ORF1ab, RdRp, N, E, S	Multiple	40-50	Yes	(Jung et al., 2020; Kudo et al., 2020; Park et al., 2020; Mancini et al., 2021)
			N-RT-qPCR	ORF1ab, RdRp, N, E, S	Both	50-120	Yes	(Wang J. et al., 2020; Yip et al., 2020; La Rosa et al., 2021)
		RT-dPCR	RT-ddPCR	ORF1ab, RdRp, N, E N	Both	70-170	Yes	(Vasudevan et al., 2021)
			RT - Chip-based dPCR		Single	80	Yes	(Poggio et al., 2021)
	Non-PCR	qSTAR Technology		ORF1a	Single	20	Yes	(In, Diagnostic and Only, 2021)

*Retrieved from the instructions for use of approved diagnostic products available in FDA website (https://www.fda.gov/medical-devices/coronavirus-disease-2019-covid-19-emergency-use-authorizations-medical-.devices/in-vitro-diagnostics-euas-molecular-diagnostic-tests-sars-cov-2).