Skip to main content
. 2022 Mar 23;12:799678. doi: 10.3389/fcimb.2022.799678

Table 6.

Depiction of distinct physicochemical routes used for performance of SARS-CoV-2 detection.

Physicochemical detection strategy Real-time or End-point Included in assay issued with EUA Source
Nucleic acids detection PCR-associated methods Optical Fluorescence Oligonucleotide Probes Taqman probes Real-time Yes* (Falzone et al., 2020)
Molecular beacons Real-time Yes* (Banada et al., 2021)
DNA-binding dyes SYBR Green, or EvaGreen Real-time No* (González-González et al., 2020; Toptan et al., 2020)
CRISPR-based SENA Both No* (Huang et al., 2020)
Eletrochemical Voltametry Intercalating redox reporters Methylene blue Real-time No* (Nunez-Bajo et al., 2020)
Isothermal amplification Optical Fluorescence Oligonucleotide probes Molecular beacons Real-time Yes* (Bhadra et al., 2020)
DNA-binding dyes SYBR Green, EvaGreen, or SYTO-82 Both No* (Ganguli et al., 2020; Garneret et al., 2021; Marinowic et al., 2021)
CRISPR-based SHERLOCK Both Yes* (Patchsung et al., 2020)
CARMEN Both No* (Ackerman et al., 2020)
DETECTR Both Yes* (Street and Francisco, 2020)
ENHANCE Both No* (Nguyen et al., 2020)
CONAN Both No* (Yoshimi et al., 2020)
FELUDA Both No* (Azhar et al., 2021)
Chemiluminescence HPA Real-time Yes* (Inc, 2021)
Colorimetry NPs + oligonucleotide probes AuNPs End-point No* (Alafeef et al., 2021)
pH indicators End-point Yes* (González-González et al., 2021)
DNA-binding dyes SYBR Green, or EvaGreen End-point No* (García-Bernalt Diego et al., 2021; Lau et al., 2021)
CRISPR-based SHERLOCK End-point No* (Patchsung et al., 2020)
Scattering of light Angle-dependent light scatter analysis Real-time No* (Day et al., 2021)
Eletrochemical detection Amperometry Naopore Target Sequencing End-Point No* (Ptasinska et al., 2021)
Voltametry Intercalating redox reporters Real-time No* (Chaibun et al., 2021)