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. 2022 Mar 23;13:822615. doi: 10.3389/fphar.2022.822615

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Copyright © 2022 Lu, Wang, Chen, Fan, Zhai, Fang, Cai, Liu, Wu and Cai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Screening and identification of the differential metabolites in the different groups. The score plot of the metabolic differences between group S and AS (A), and between group I and AI (B) by Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) in both positive and negative ion modes. The heat maps of different metabolites in each sample from the four different groups in both positive and negative ion mode (C). The ionic strengths of both the positive and negative modes of the different metabolites between group S and AS (D), and between group I and AI (E) by variable importance in projection (VIP) generated after OPLS-DA. The subsequent Venn diagram in the negative ion mode (F). The mean relative abundance of five known differential metabolites in the different groups (G). S: sham; AS: ARB + sham; I: injury; AI: ARB + injury.