TABLE 2.
Organism/plasmid | ORF includeda
|
Novobiocic acid synthetase activity (pkat/mg of protein)b | ||||||
---|---|---|---|---|---|---|---|---|
novH | novI | novJ | novK | novL | novM | novN | ||
S. spheroidesc | + | + | + | + | + | + | + | 53.97 |
S. lividans TK24/pSL3(+): | ||||||||
Full assay | + | + | + | + | + | + | + | 9.07 |
ring A | <0.014 | |||||||
ring B | <0.014 | |||||||
ATP | <0.014 | |||||||
Heat-denaturated enzyme | <0.014 | |||||||
S. lividans TK24/pMS73 | + | + | + | + | + | 0 | 0 | 229.63 |
S. lividans TK24/pMS75 | + | + | + | + | 0 | 0 | 0 | <0.014 |
S. lividans TK24/pMS71 | + | + | + | 0 | 0 | 0 | 0 | <0.014 |
S. lividans TK24/pMS65 | + | 0 | 0 | 0 | 0 | 0 | 0 | <0.014 |
S. lividans TK24/pUWL201d | 0 | 0 | 0 | 0 | 0 | 0 | 0 | <0.014 |
See Fig. 2 for the exact length of the plasmid insert. +, ORF is included; 0, ORF is not included.
Data are mean values from experiments with two independent transformants, with each assay performed in duplicate.
S. spheroides is the producer of novobiocin and contains the novobiocin biosynthetic genes in the genomic DNA.
Empty vector.