TABLE 2.

Heterologous expression of novobiocic acid synthetase in S. lividans TK24

Organism/plasmid	ORF includeda							Novobiocic acid synthetase activity (pkat/mg of protein)b
	novH	novI	novJ	novK	novL	novM	novN
S. spheroidesc	+	+	+	+	+	+	+	53.97
S. lividans TK24/pSL3(+):
Full assay	+	+	+	+	+	+	+	9.07
ring A								<0.014
ring B								<0.014
ATP								<0.014
Heat-denaturated enzyme								<0.014
S. lividans TK24/pMS73	+	+	+	+	+	0	0	229.63
S. lividans TK24/pMS75	+	+	+	+	0	0	0	<0.014
S. lividans TK24/pMS71	+	+	+	0	0	0	0	<0.014
S. lividans TK24/pMS65	+	0	0	0	0	0	0	<0.014
S. lividans TK24/pUWL201d	0	0	0	0	0	0	0	<0.014

^aSee Fig. 2 for the exact length of the plasmid insert. +, ORF is included; 0, ORF is not included. 

^bData are mean values from experiments with two independent transformants, with each assay performed in duplicate. 

^cS. spheroides is the producer of novobiocin and contains the novobiocin biosynthetic genes in the genomic DNA. 

^dEmpty vector.